Anti-V-ATPase H ATP6V1H Antibody

Product Name

Anti-V-ATPase H ATP6V1H Antibody

View all ATP6V1H Antibodies

SKU/Catalog Number

A11008-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-V-ATPase H ATP6V1H Antibody catalog # A11008-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-V-ATPase H ATP6V1H Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11008-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human V-ATPase H

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11008-2 is reactive to ATP6V1H in Human, Mouse

Applications

A11008-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.883kDa

Background of ATP6V1H

Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system By similarity. Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.

Liu B., Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Lu X., Immunity 8:647-656(1998).
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Lai C.-H., Genome Res. 10:703-713(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-V-ATPase H ATP6V1H Antibody A11008-2

Western Blot (WB) analysis of specific cells using V-ATPase H polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ATP6V1H (Source: Uniprot.org, NCBI)

Alternative Names

ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H; ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H; CGI-11; MSTP042; NBP1; Nef-binding protein 1; Protein VMA13 homolog; SFD; SFDalpha; SFDbeta; vacuolar ATP synthase subunit H; vacuolar ATPase subunit H; vacuolar proton pump H subunit; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; V-ATPase 50/57 kDa subunits; V-ATPase H subunit; V-ATPase subunit H; VMA13; V-type proton ATPase subunit H ATP6V1H CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13 ATPase H+ transporting V1 subunit H V-type proton ATPase subunit H|ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H|V-ATPase 50/57 kDa subunits|V-ATPase subunit H|nef-binding protein 1|protein VMA13 homolog|vacuolar ATP synthase subunit H|vacuolar ATPase subunit H|vacuolar proton pump subunit H|vacuolar proton pump subunit SFD

For more info on ATP6V1H, check out the ATP6V1H Infographic

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